I love strawberries! Fresh ones are always best to use if you’re serving the trifle right away. If it’s going to sit for any amount of time, the strawberries will get a little soggy anyway and then it doesn’t matter if you used frozen or fresh. Frozen are usually cheaper in my experience, unless strawberries are in season.
If you divide the entire recipe into 12, it’ll be 4 points each. Estimating about ¾ cup (maybe a little more) per serving.